Screening and characterization of antioxidant, anti-aging, and anti-microbial activity of herbal extracts in Northern Thailand

Background: Aging is a process caused by oxidants and aging-related enzymes. Therefore, the inhibition of these processes can exacerbate anti-aging agents. This study aimed to evaluate antioxidant activities leaf extracts from seven herbs used in traditional Thai herbal remedies. Methods: Researchers assessed total levels phenolic content (TPC), antioxidant, anti-collagenase, anti-tyrosinase activity using colorimetric methods. Cytotoxicity effects were determined MTT assays, matrix metalloproteinases(MMPs) secretion was through gelatin zymography. In addition, inhibitory on growth microorganisms examined disc diffusion broth microdilution method.Results: TPC ranged between 48.68–440.91 mg GAE/g ethanolic extracts. High against ABTS radicals detected P. granatum, emblica, guajava, T. bellirica, chebula, while high DPPH neutralization appeared M. coreia, granatum. FRAP assays significantly reduced power chebula guajava exhibited highest effect H2O2induced ROS production. E. hygrophilus tyrosinase collagenase activities. gaujava, bellirica shown inhibit MMP-2 fibroblast cells. All concentrations non-toxic P.granatum could acnes, coli, S. aureus. Conclusion: Preliminary studies showed that have antioxidants, anti-aging, anti-bacterial activities, as well active ingredients suitable for cosmetic products.Keywords: